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KMID : 1007520130220041055
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Food Science and Biotechnology
2013 Volume.22 No. 4 p.1055 ~ p.1061
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In vitro Antioxidant Activities of Laccaic Acids and Its Aluminum Lake
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Ampa Jimtaisong
Rudeemars Janthadee
Taveeporn Nakrit
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Abstract
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Antioxidant activities of laccaic acids and its aluminum lake were investigated by DPPH assay, reducing power, and thiocyanate method. The DPPH assay, which used for the reaction kinetic of samples by measuring the ability of antioxidants on reacting with DPPH in a function of time, showed that 0.1, 0.2, and 0.5 mg/mL laccaic acids and 0.5 and 1.25 mg/mL aluminum lake possessed intermediate behavior since they reacted slowly and reach a steady state within 30 min. Laccaic acids of 1.0 mg/mL and aluminum lac lake at concentrations of 2.5 and 5.0 mg/mL reacted much slower and took longer time to reach a steady state thus possessed a slow kinetic behavior. The DPPH assay also showed that antioxidative activity of laccaic acids (EC50=0.38mg/mL) is higher than the aluminum lake (EC50=1.63 mg/mL) and butylated hydroxytoluene (EC50=0.57 mg/mL) but lower than ascorbic acid (EC50= 0.14 mg/mL), and gallic acid (EC50=0.05 mg/mL). The reducing power assay indicated that laccaic acids had greater reducing power than aluminum lake. While, % inhibition of lipid peroxidation of laccaic acids (29.9%) was lower than the lac lake (43.8%).
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KEYWORD
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laccaic acid, aluminum lake, antioxidant activity, DPPH, lipid peroxidation
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